For reference the fast calling mode was ~8 minutes. So from the above we see in high accuracy mode it take the Xavier ~41 minutes to complete the base calling using the default configuration files. Model file: /opt/ont/guppy/data/template_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.jsn ONT Guppy basecalling software version 3.4.1+213a60d0Ĭonfig file: /opt/ont/guppy/data/dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg Guppy_basecaller -disable_pings -compress_fastq -c dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg -fast5_out -i flongle_fast5_pass/ -s flongle_hac_fastq -x 'auto ' -recursive $ guppy_basecaller -compress_fastq -c dna_r9.4.1_450bps_modbases_dam-dcm-cpg_hac.cfg -i flongle_fast5_pass/ -s flongle_hac_fastq -x 'auto' -recursive